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Vol. 81 (LXXXI) 101-113
| DETERMINATION OF PIPERAZINE DERIVATIVES IN “LEGAL HIGHS” | |
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Bogumiła BYRSKA, Dariusz ZUBA, Ro man STANASZEK
Abstract The paper pres ents the results of the development of chromatographic methods for determination of piperazine derivatives, including 1-benzylpiperazine (BZP), 1-(3-chlorphenyl)piperazine (mCPP), N-(3-methylbenzyl)piperazine (MeBP), 1-(2-methoxy-phenyl)piperazine (MeOPP), 1-methyl-3-phenylpiperazine (MeP) and 1-(3-trifluoromethylphenyl)piperazine(TFMPP). Qual i ta tive anal y sis of piperazine derivatives was carried out by gas chromatography coupled to mass spectrometry (GC-MS), while quantitative analysis was performed by high performance liquid chro matography with diode array detection (HPLC-DAD). Mass spectra of piperazine derivatives are characteristic, which allows their identification on the basis of ions with the following m/z values: BZP – 91, 134, 176, 56; mCPP – 154, 196, 138, 57; MeBP – 105, 148, 190, 56; MeOPP – 150, 192,135, 120; MeP – 58, 104, 176, 43; TFMPP – 188, 230, 172, 145. Optimization of HPLC conditions allowed separation of all analytes in less than 4 min (total analysis time was 9 min). Limits of quantification (LOQ) of individual compounds ranged from 0.125 mg/ml to 0.5 mg/ml. The HPLC method was linear over the entire range of tested concentrations. Precision and accuracy were studied at three concentration levels: 5, 20 and 50 mg/ml. The values of both analytical parameters were below 4% in most cases. The developed chromatographic (GC-MS, HPLC) methods allow qualitative and quantitative analysis of piperazine derivatives in sam plesseized on the illicit drug market and in so-called “legal highs”. Key words “Legal highs”; Piperazine derivatives; GC-MS; HPLC-DAD |
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